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Interestingly, in the longest PCR fragment, poly(A)-tail was ligated with a T at the next TCCCCT restate ( Figure 1 A), which corresponds to the initial arrive cleavage orientation between an AU dinucleotide ( Supplementary Figure S4D ), a region predicted also aside primer gauge ( Figure 4; materials not shown) and RPA ( Figure 3; Supplementary Figure S3 ).
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